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1993•FEMS Microbiology LettersOpen access

Fast classification of plant-associated bacteria in the Xanthomonas genus

Mesa de Agroecologia y Suelo MAES

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Abstract

A rapid method for identifying Xanthomonas bacteria was developed, based on specific PCR amplification. In all Xanthomonas pathovars tested, a single 16S rDNA fragment of 480 bp was produced. Phytobacteria from other genera showed other fragment patterns on agarose gel electrophoresis. This Xanthomonas identification system was used to screen Xanthomonas-related organisms, newly isolated phytobacteria and bacterial populations in wheat seed extracts.

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A rapid method for identifying Xanthomonas bacteria was developed, based on specific PCR amplification. In all Xanthomonas pathovars tested, a single 16S rDNA fragment of 480 bp was produced. Phytobacteria from other genera showed other fragment patterns on agarose gel electrophoresis. This Xanthomonas identification system was used to screen Xanthomonas-related organisms, newly isolated phytobacteria and bacterial populations in wheat seed extracts.

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Available abstract

A rapid method for identifying Xanthomonas bacteria was developed, based on specific PCR amplification. In all Xanthomonas pathovars tested, a single 16S rDNA fragment of 480 bp was produced. Phytobacteria from other genera showed other fragment patterns on agarose gel electrophoresis. This Xanthomonas identification system was used to screen Xanthomonas-related organisms, newly isolated phytobacteria and bacterial populations in wheat seed extracts.

Key concepts: Xanthomonas, Biology, Bacteria, Agarose gel electrophoresis, Microbiology, 16S ribosomal RNA, Polymerase chain reaction, Pseudomonadaceae

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