2021•BioScientific ReviewOpen access

Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

Malik Nawaz Shuja

Abstract

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

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Available abstract

Key concepts: Kanamycin, Selectable marker, Transformation (genetics), Biology, Biotechnology, Selection (genetic algorithm), Genetically modified crops, Agrobacterium