1969•Agricultural and Biological ChemistryOpen access
Studies on Amino Acid Sequence Determination from N-Terminal of Peptide by Methylthiohydantoin
Tateo Suzuki, Hiroshi Kawauchi, Hiroshi Meguro, Katura Tuzimura
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Abstract
Methylisothiocyanatereacts with the amino group of amino acid in alkaline solution to give methylthiocarbamyl amino acid.This is converted to the methylthiohydantoin derivative (MTH-amino acid) by subsequent acidification.Gas chromatographical identification of 20 amino acids were examined by making MTH-amino acids.Among them, ten amino acids (glycine, alanine, valine, leucine, iso leucine, proline, methionine, phenylalanine, serine and threonine) were successfully identified.Aspartic acid and glutamic acid could be identified as the methyl esters of the MTHamino acids.
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