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1983•FEMS Microbiology LettersOpen access

A novel shuttle cloning vector for the cyanobacterium Anacystis nidulans

S Gendel

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Abstract

Shuttle cloning vectors for use with the cyanobacterium Anacystis nidulans and Escherichia coli were constructed by combining an endogenous A. nidulans plasmid with an E. coli vector containing a 14 site non-symmetrical polylinker. The resulting plasmids, designated pPLAN B1 and pPLAN B2, transform A. nidulans with high efficiency and contain 7 unique restriction enzyme sites suitable for cloning.

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Shuttle cloning vectors for use with the cyanobacterium Anacystis nidulans and Escherichia coli were constructed by combining an endogenous A. nidulans plasmid with an E. coli vector containing a 14 site non-symmetrical polylinker. The resulting plasmids, designated pPLAN B1 and pPLAN B2, transform A. nidulans with high efficiency and contain 7 unique restriction enzyme sites suitable for cloning.

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Available abstract

Shuttle cloning vectors for use with the cyanobacterium Anacystis nidulans and Escherichia coli were constructed by combining an endogenous A. nidulans plasmid with an E. coli vector containing a 14 site non-symmetrical polylinker. The resulting plasmids, designated pPLAN B1 and pPLAN B2, transform A. nidulans with high efficiency and contain 7 unique restriction enzyme sites suitable for cloning.

Key concepts: Shuttle vector, Multiple cloning site, Cloning (programming), Plasmid, Cloning vector, Escherichia coli, Molecular cloning, Biology

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