Cell Counting and Viability Measurements

The growth of mammalian cells in culture can be monitored by a number of parameters related to the increase of cellular biomass over time. The simplest method is by cell counting at regular intervals. In routine cultures this would be performed once a day, which corresponds to the approximate doubling time of mammalian cells during the exponential growth phase. This would establish an overall growth profile of a culture. More frequent counts would be required to follow more subtle changes that may, for example, be associated with the cell growth cycle. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.