2003•Current Protocols in Cell BiologyRequires access

Two‐Photon Excitation Microscopy for the Study of Living Cells and Tissues

Jonathan V. Rocheleau, David W. Piston

Abstract

Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages in three-dimensional and deep tissue imaging. This unit will describe the basic physical principles of two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth, and the ability to initiate localized photochemistry.

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Available abstract

Key concepts: Two-photon excitation microscopy, Microscopy, Excitation, Confocal microscopy, Phototoxicity, Materials science, Optics, Multiphoton fluorescence microscope