The Fate of Glucose in Strains S288C and S173‐6B of the Yeast Saccharomyces cerevisiae

Intracellular metabolic flux has been investigated in two strains of Saccharomyces cerevisiae grown into stationary phase under both glucose-repressed and glucose-derepressed conditions. By employing a variety of simple methodologies (manometry, enzymatic analysis and colorimetric analysis) we have been able to identify and quantitate carbon flow from glucose without the need for isotopically labelled substrate. We can account for 88–98% (depending on strain and growth conditions) of the carbon products of glucose metabolism under both glycolytic and oxidative conditions as ethanol (27–40%), carbon dioxide (15–26%), acetate (2–3%), glycerol (5–11%), glycogen (5–13%) and trehalose (9–39%).©1997 John Wiley & Sons, Ltd.