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2017•Unpublished venueOpen access

Electrophoretic Mobility Shift Assay (EMSA) Using IRDye® Oligonucleotides v2

LI-COR Biosciences, Bandana Maharjan

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Abstract

This is a sample Mobility Shift Protocol (NFκB).Each oligo labeled with IRDye 700 provided by LI-COR® Biosciences for EMSA reactions will have an optimized protocol to measure the protein-DNA interaction. See the specific EMSA oligo pack insert for more information. As an example, the NFκB protein-DNA interaction will be described in this protocol. Please refer to your manual to confirm that this protocol is appropriate for the applications compatible with your Odyssey Imager model.

About this research paper

What this paper is about

This is a sample Mobility Shift Protocol (NFκB).Each oligo labeled with IRDye 700 provided by LI-COR® Biosciences for EMSA reactions will have an optimized protocol to measure the protein-DNA interaction. See the specific EMSA oligo pack insert for more information. As an example, the NFκB protein-DNA interaction will be described in this protocol. Please refer to your manual to confirm that this protocol is appropriate for the applications compatible with your Odyssey Imager model.

Why it matters

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Key contribution

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Method / approach

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Available abstract

This is a sample Mobility Shift Protocol (NFκB).Each oligo labeled with IRDye 700 provided by LI-COR® Biosciences for EMSA reactions will have an optimized protocol to measure the protein-DNA interaction. See the specific EMSA oligo pack insert for more information. As an example, the NFκB protein-DNA interaction will be described in this protocol. Please refer to your manual to confirm that this protocol is appropriate for the applications compatible with your Odyssey Imager model.

Key concepts: Electrophoretic mobility shift assay, Oligonucleotide, Chemistry, DNA, Protocol (science), Electrophoresis, Insert (composites), Biophysics

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