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2000•Medical and Pediatric OncologyRequires access

Deletion mapping of 14q32 in human neuroblastoma defines an 1,100‐kb region of common allelic loss

Masato Hoshi, Hiromi O. Shiwaku, Yutaka Hayashi, Yasuhiko Kaneko, Akira Horii

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Abstract

Background We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH. Procedure The smallest region of overlap (SRO) was identified between D14S62 and D14S987. Results There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb. Conclusions Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB. Med. Pediatr. Oncol. 35:522–525, 2000. © 2000 Wiley-Liss, Inc.

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Background We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH. Procedure The smallest region of overlap (SRO) was identified between D14S62 and D14S987. Results There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb. Conclusions Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB. Med. Pediatr. Oncol. 35:522–525, 2000. © 2000 Wiley-Liss, Inc.

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Available abstract

Background We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH. Procedure The smallest region of overlap (SRO) was identified between D14S62 and D14S987. Results There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb. Conclusions Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB. Med. Pediatr. Oncol. 35:522–525, 2000. © 2000 Wiley-Liss, Inc.

Key concepts: Loss of heterozygosity, Contig, Neuroblastoma, Microsatellite, Genetics, Allele, Carcinogenesis, Biology

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Deletion mapping of 14q32 in human neuroblastoma defines an 1,100‐kb region of common allelic loss — Research Paper | ScholarLens