Photometrical and dosimetrical measurements for Photodynamic Diagnosis and Therapy with ALA and h-ALA induced PpIX

The quantification of the induced fluorescence in tumor tissue is important to design optical equipment for photodynamic diagnosis (PDD). The fluorescence intensities of Protoporphyrin IX (PpIX) solved in Dimethylsulfoxid and induced via application of Aminolevulinic Acid in cells, cultivated in the hen's eggs model, have been measured photometrically. With an optimized CCD-camera-systems fluorescent tumor areas were detected before and after photodynamic therapy (PDT). The ratio of dead cells was detected by staining with trypan blue after PDT. The measurements were carried out with various energy densities at the time of maximal PpIX-enrichement.