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1995•FEMS Microbiology LettersOpen access

Construction of an integrative shuttle vector for Zymomonas mobilis

Osvaldo Daniel Delgado

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Abstract

An integrative shuttle vector, pZMOCPI, was constructed by ligating EcoRV digests of the plasmid cloning vector pBluescript and pZMPI, a cryptic plasmid of Zymomonas mobilis PROM Al. The 7.2-kb plasmid pZMOCPI replicated in Escherichia coli and could also be transferred from this host by electroporation to Z. mobilis ATCC 29191. The transformants were selected by ampicillin resistance. The integrative characteristic was detected by hybridization in situ. The vector was stably maintained in Z. mobilis after 200 generations without selective pressure.

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An integrative shuttle vector, pZMOCPI, was constructed by ligating EcoRV digests of the plasmid cloning vector pBluescript and pZMPI, a cryptic plasmid of Zymomonas mobilis PROM Al. The 7.2-kb plasmid pZMOCPI replicated in Escherichia coli and could also be transferred from this host by electroporation to Z. mobilis ATCC 29191. The transformants were selected by ampicillin resistance. The integrative characteristic was detected by hybridization in situ. The vector was stably maintained in Z. mobilis after 200 generations without selective pressure.

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Available abstract

An integrative shuttle vector, pZMOCPI, was constructed by ligating EcoRV digests of the plasmid cloning vector pBluescript and pZMPI, a cryptic plasmid of Zymomonas mobilis PROM Al. The 7.2-kb plasmid pZMOCPI replicated in Escherichia coli and could also be transferred from this host by electroporation to Z. mobilis ATCC 29191. The transformants were selected by ampicillin resistance. The integrative characteristic was detected by hybridization in situ. The vector was stably maintained in Z. mobilis after 200 generations without selective pressure.

Key concepts: Zymomonas mobilis, Shuttle vector, Plasmid, Electroporation, Multiple cloning site, Cloning vector, Biology, Escherichia coli

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