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Isolation of Photosystem II Reaction Center Complexes From Plants

Michael Seibert, Inmaculada Yruela, Rafael Picorel

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Abstract

Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cyt b559 photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material is also described. Criteria to assess the purity of photosystem II (PSII) reaction center preparations are presented, and problems associated with each of the isolation procedures are discussed.

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What this paper is about

Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cyt b559 photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material is also described. Criteria to assess the purity of photosystem II (PSII) reaction center preparations are presented, and problems associated with each of the isolation procedures are discussed.

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Available abstract

Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cyt b559 photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material is also described. Criteria to assess the purity of photosystem II (PSII) reaction center preparations are presented, and problems associated with each of the isolation procedures are discussed.

Key concepts: Photosynthetic reaction centre, Photosystem II, Isolation (microbiology), Chemistry, Photochemistry, Chlorophyll, Photosynthesis, Chlorophyll a

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