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1988•FEMS Microbiology LettersOpen access

A system for the inducible secretion of proteins from Bacillus subtilis during logarithmic growth

A Edelman

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Abstract

A Bacillus subtilis-Escherichia coli shuttle vector was constructed containing the B. subtilis levansucrase gene promoter and region encoding its signal sequence. A site for the restriction enzyme NaeI was included to facilitate precise translational fusions to the DNA encoding the levansucrase signal sequence. Fusions of TEM β-lactamase to this construct displayed sucrose-inducible expression and secretion of B. subtilis.

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A Bacillus subtilis-Escherichia coli shuttle vector was constructed containing the B. subtilis levansucrase gene promoter and region encoding its signal sequence. A site for the restriction enzyme NaeI was included to facilitate precise translational fusions to the DNA encoding the levansucrase signal sequence. Fusions of TEM β-lactamase to this construct displayed sucrose-inducible expression and secretion of B. subtilis.

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Available abstract

A Bacillus subtilis-Escherichia coli shuttle vector was constructed containing the B. subtilis levansucrase gene promoter and region encoding its signal sequence. A site for the restriction enzyme NaeI was included to facilitate precise translational fusions to the DNA encoding the levansucrase signal sequence. Fusions of TEM β-lactamase to this construct displayed sucrose-inducible expression and secretion of B. subtilis.

Key concepts: Levansucrase, Bacillus subtilis, Shuttle vector, Signal peptide, Escherichia coli, Biology, Gene, Expression vector

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