Karla L. H. Feijs
Abstract
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This is our calcium phosphate transfection protocol. It always works with HEK293T cells, for other lines the transfection efficiency depends on their source: some HeLa and U2OS clones can be transfected using CaPO4, however most cannot. We mostly use this method to transfect HEK293T cells for lentivirus production.
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This is our calcium phosphate transfection protocol. It always works with HEK293T cells, for other lines the transfection efficiency depends on their source: some HeLa and U2OS clones can be transfected using CaPO4, however most cannot. We mostly use this method to transfect HEK293T cells for lentivirus production.
Key concepts: Transfection, HEK 293 cells, HeLa, Cell biology, Calcium, Molecular biology, Cell culture, Chemistry