1996FEMS Microbiology LettersOpen access

Relationship between outer membrane protein and lipopolysaccharide profiles and serotypes of enterotoxigenic Escherichia coli isolated in Brazil

Lucília Sanae Nishimura

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Abstract

The electrophoretic profiles of outer membrane proteins and lipopolysaccharide of sixty-five enterotoxigenic Escherichia coli of different serotypes and virulence-associated factors, toxin and colonization factors were determined. A close relationship between serotype and outer membrane protein and lipopolysaccharide patterns could be observed. No correlation could be found between the electrophoretic profiles and the expression of virulence-associated factors. The observed homogeneity of outer membrane protein and lipopolysaccharide profiles suggested the presence of only a few clones in the samples studied, and supported the use of outer membrane protein and lipopolysaccharide analysis as a useful epidemiological tool in the characterization of enterotoxigenic Escherichia coli isolates.

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The electrophoretic profiles of outer membrane proteins and lipopolysaccharide of sixty-five enterotoxigenic Escherichia coli of different serotypes and virulence-associated factors, toxin and colonization factors were determined. A close relationship between serotype and outer membrane protein and lipopolysaccharide patterns could be observed. No correlation could be found between the electrophoretic profiles and the expression of virulence-associated factors. The observed homogeneity of outer membrane protein and lipopolysaccharide profiles suggested the presence of only a few clones in the samples studied, and supported the use of outer membrane protein and lipopolysaccharide analysis as a useful epidemiological tool in the characterization of enterotoxigenic Escherichia coli isolates.

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Available abstract

The electrophoretic profiles of outer membrane proteins and lipopolysaccharide of sixty-five enterotoxigenic Escherichia coli of different serotypes and virulence-associated factors, toxin and colonization factors were determined. A close relationship between serotype and outer membrane protein and lipopolysaccharide patterns could be observed. No correlation could be found between the electrophoretic profiles and the expression of virulence-associated factors. The observed homogeneity of outer membrane protein and lipopolysaccharide profiles suggested the presence of only a few clones in the samples studied, and supported the use of outer membrane protein and lipopolysaccharide analysis as a useful epidemiological tool in the characterization of enterotoxigenic Escherichia coli isolates.

Key concepts: Bacterial outer membrane, Lipopolysaccharide, Microbiology, Virulence, Escherichia coli, Enterotoxigenic Escherichia coli, Serotype, Biology

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