1930•Experimental Biology and MedicineRequires access
Precipitin Variants
J. L. Azevedo, W. H. Manwaring
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Abstract
Anti-horse precipitin withdrawn from a rabbit 10 days after injection of the final immunizing dose of horse proteins gives precipitin graphs suggesting a 100% 17th-day retention of intravenously injected horse proteins in the canine circulation, with marked denaturization of these proteins. Parallel tests with precipitin withdrawn 23 days after the final immunizing dose, give graphs suggesting but 1% horse protein retention with no horse protein denaturization. Our conventional assumption that the specific precipitin is biochemically identical at all stages of sensitization and immunization with the same antigen evidently requires further study. This report gives the maximum difference observed in 12 parallel titrations of parenteral horse protein derivatives with about 20 different rabbit antisera.