Structure of a novel metabolite from deoxynivalenol, a trichothecene mycotoxin, in animals.

Deoxynivalenol(DON), 3a,7a,15-trihydroxy-12,13-epoxytrichothec-9-en-8-one, is a toxic metabolite such Fusarium spp. as F.graminearuml) and F. culmorum.2) It is commonly found together with nivalenol in wheat and barley grains in Japan,3) and is also one of the most important trichothecene mycotoxins occurring naturally in several cereals and animal feed in other countries.4)As a result of the consumption of these contaminated agricultural products, animals, even man, are suffering from sublethal toxicoses including emesis, diarrhea and feed refusal.5*However, the metabolic fate of the toxin is still unknown.In this paper, we describe the elimination of DONin rats and the structural elucidation of a novel metabolite found in the excreta.DONwas dissolved in 10% aqueous ethanol and intubated to Wistar male rats weighing 240~245g at a single dose of 6mg/kg.Urine and feces samples were collected every 12hr