Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non‐artificial conditions
Elke Nevoigt, Anne Faßbender, Ulf Ståhl
Abstract
Elke Nevoigt, Anne Faßbender, Ulf Ståhl
Abstract
Transformants of bakers' yeast (Saccharomyces cerevisiae) can be generated when non-growing cells metabolize sugars (without additional nutrients) in the presence of plasmid DNA. These results suggest that there is a mechanism by which DNA can naturally be taken up by the yeast cell. Natural transformation does not take place in common complete or minimal yeast culture media such as YPD and YNB. The starvation conditions used in our experiments thus seem to be an important prerequisite for such transformation events. Copyright © 2000 John Wiley & Sons, Ltd.
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Transformants of bakers' yeast (Saccharomyces cerevisiae) can be generated when non-growing cells metabolize sugars (without additional nutrients) in the presence of plasmid DNA. These results suggest that there is a mechanism by which DNA can naturally be taken up by the yeast cell. Natural transformation does not take place in common complete or minimal yeast culture media such as YPD and YNB. The starvation conditions used in our experiments thus seem to be an important prerequisite for such transformation events. Copyright © 2000 John Wiley & Sons, Ltd.
Key concepts: Saccharomyces cerevisiae, Yeast, Biology, Transformation (genetics), Plasmid, DNA, Saccharomyces, Biochemistry