The Pollen Grain Wall as a Site for Passage of Lanthanum in Tomato, Lycopersicon esculentum (Solanaceae)

We studied the response of the pollen grain wall to the passage of materials from the external environment. Pollen grains of Lycopersicon esculentum, a species not previously investigated in similar studies, were used to determine whether the routes of passage through the pollen wall are a characteristic of a given species. We found lanthanum deposits on the cell wall surface, inside microchannels crossing tectum, and in the infratectum. Lanthanum was also seen as a fine precipitate homogenously scattered within the substance of the exine. In the apertural region of the intine, electron-dense deposits filled the tubulations of the intine oncus, whereas the intine matrix was free of precipitate. In the cytoplasm, precipitate was observed inside small vesicles frequently located near or in contact with the plasmalemma. Our findings were similar to those reported in other species, and were not influenced by the fixative or culture medium used to incorporate the tracer element. We noted a relation between the route of passage and the stage of pollen grain maturation. In less mature grains (midbicellular pollen), electron-dense deposits were more abundant than in more mature grains, probably because apertural regions were blocked as routes of passage.