Pengaruh Pemberian Susu Skim dengan Pengencer Tris Kuning Telur terhadap Daya Tahan Hidup Spermatozoa Sapi pada Suhu Penyimpanan 5ºC

The objective of the study was to determine the effect of skim milk and Tris yolk extender on the viability bovine spermatozoa at 5 ºC. This study used Simmental bull semen. The design used was Randomized Design Group (RAK) with 5 treatments and 5 groups of ejaculate. The treatments were: P0 = skim milk 100% without Tris yolk extender; P1 = 95% skim milk + 5% Tris yolk extender, P2 = 90% skim milk + Tris yolk extender 10%, P3 = skim milk 85% + Tris yolk extender 15%, P4 = skim milk 80% + Tris yolk extender 20%. The observed variables were the percentage of live spermatozoa and the motility of spermatozoa after storage for 2 days at 5ºC. Skim milk with Tris yolk extender affect the viability and motility of spermatozoa stored at 5ºC (P<0.01). Provision of extender up to 15% gave the best results and suppressed the decline in the viability of spermatozoa storaged for 2 days at 5ºC. Key words: skim milk, spermatozoa, Tris yolk extender, viability