SYNTHESIS OF TRITIUM LABELLED THYMIDINE AND URIDINE

This study present tritium labeling methods for uridine and thymidine in the methyl position. The synthesis of tritiated thymidine has been performed using two different methods: catalytic hydrogenation of brominated derivative and respectively of hydroxymethyl deoxiuridine. The tritiated uridine has been synthesized by catalytic hydrogenation of 5-I Uridine. The labelled compounds were purified by Thin Layer Chromatography and characterized by determination of radioactive concentration, chemical concentration and radiochemical purities. The obtained tritiated thymidine and uridine have been used for in vitro studies for evaluation of radiation effects in cell cultures.