2012•Methods in molecular biologyRequires access

Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

Claudia Arndt, Stefanie Koristka, Anja Feldmann, Holger Bartsch, Michael Philipp Bachmann

Open publisher page 29 citations

Abstract

Over the past, a series of staining procedures for proteins were published. The most commonly used staining dye for proteins is still Coomassie-Brilliant Blue. The major reason is Coomassie-Brilliant Blue staining is simple, fast, and sensitive. As Coomassie-Brilliant Blue is almost insoluble in water, a series of procedures including colloidal aqueous procedures were described.

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What this paper is about

Over the past, a series of staining procedures for proteins were published. The most commonly used staining dye for proteins is still Coomassie-Brilliant Blue. The major reason is Coomassie-Brilliant Blue staining is simple, fast, and sensitive. As Coomassie-Brilliant Blue is almost insoluble in water, a series of procedures including colloidal aqueous procedures were described.

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Available abstract

Over the past, a series of staining procedures for proteins were published. The most commonly used staining dye for proteins is still Coomassie-Brilliant Blue. The major reason is Coomassie-Brilliant Blue staining is simple, fast, and sensitive. As Coomassie-Brilliant Blue is almost insoluble in water, a series of procedures including colloidal aqueous procedures were described.

Key concepts: Coomassie Brilliant Blue, Staining, Brilliant green, Chromatography, Chemistry, Medicine, Pathology, Organic chemistry

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