Gentamicin Protection assay (Intracellular Survival Assay) for Salmonella Typhimurium/Typhi v1

Salmonella invades the epithelial cells by inducing bacterial uptake, whereas phagocytes readily take up the bacteria by phagocytosis. Intracellular Salmonella is resistant to gentamicin, but extracellular bacteria are susceptible to the antibiotic, therefore, adhesion, invasion, and bacterial replication can be measured using this protocol. Inside the cells, Salmonella proliferates and the rate of proliferation can be determined by enumerating the bacterial CFU from the infected cells.