REPRODUCTION OF THE RABIES VIRUS OF “RV-97” STRAIN IN THE SUSPENSION CULTURE OF VNK-21 / SUSP / ARRIAH AND VNK -21 / 2-17 CELLS

The study of new suspension cell lines for rabies virus cultivation is a topical area of biotechnology. The article presents results of specification of sensitivity of BNK-21 / SUSP / ARRIAH subline to the rabies virus of “RV-97” strain. A comparison of two highly potential suspension cell cultures of VNK 21 for production of viral material with a high titer of infectious activity is shown [1]. The estimation of the titers of the rabies virus of “RV-97” strain (lg’T, CCID 50 / cm3) was carried out, the titers were established after 48 hours of cultivation in the suspension subline of VNK -21 cells, according to the cell suspension concentration and the multiplicity of infection. Suspensions of VNK-21 cells with concentrations of 700 and 1000 (thousand cells / cm³) were tested. The multiplicity of infection was 0.01; 0.1 and 0.5 CCID 50 / cell. It was found that VNK-21 / SUSP / ARRIAH subline at a concentration of 1 mln. cells / cm³ has advantages over VNK-21 / 2-17 subline and allows, at a multiplicity of infection of 0.1 CCID 50 / cell, to achieve the accumulation of the rabies virus of “RV-97” strain up to 8.25 lg CCID 50 / cm3. The listed conditions were considered suitable for cultivation of this strain. Statistical analysis of the data for substantiation of the shortest cultivation time, which allows to obtain the largest virus yield was carried out. It was determined that the peak titer value, established after 48 hours of cultivation (lg’T48 = 8.250), significantly exceeded all previous values. However, there were no significant differences from the peak estimate (p> 0.05) in subsequent samples (lg’T58 = 7.917; lg’T72 = 7.833).