Morphological and molecular identification of Sarcocystis sp. from the little grebe, Tachybaptus ruficollis (Aves: Podicipediformes), for the first time in Egypt

Abstract Background In Egypt, studies of sarcocystosis in migratory and wading birds specially Podicipediformes are scarce. Therefore, the occurrence of Sarcocystis spp. in the little grebe, Tachybaptus ruficollis (Podicipediformes: Podicipdidae) was investigated in Qena province, Upper Egypt. During the period from September 2017 to March 2018, muscle specimens of esophagus from 25 little grebes were examined for the occurrence of Sarcocystis spp. using light microscopy, transmission electron microscopy (TEM) and molecular tools including conventional PCR and phylogenetic analysis with both 18S rRNA and 28S rRNA genetic loci. Results Sarcocystis spp. were identified in 84.0% (21/25) of the T. ruficollis esophageal muscle samples by morphological analysis; each was long, ribbonlike and extended along the muscle fibers. TEM revealed a characteristic thin and wavy cyst wall and an undulating vacuolar membrane with villar protrusions of slightly variant sulci and gyri, as well as several septated compartments filled with bradyzoites. The applied 18S rRNA and 28S rRNA were not enough variable for the identification of Sarcocystis species. Conclusion This study is the first to report the occurrence of Sarcocystis sp. in little grebes in Egypt. Further studies are required to identify Sarcocystis spp. in various wild birds by ITS1 region in Egypt. Graphical abstract