2008•Unpublished venueRequires access
Electrophoretic Techniques
Reiner Westermeier, Tom Naven, Hans‐Rudolf Höpker
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Abstract
This chapter contains sections titled: The Principle of Electrophoresis and Some Methodological Background Free Flow Electrophoretic Methods Gels for Electrophoretic Techniques Electroendosmosis Effects Polyacrylamide Gel Electrophoresis The Polyacrylamide Gel SDS Polyacrylamide Gel Electrophoresis Blue Native Electrophoresis Cationic Detergent Electrophoresis Blotting Electrophoretic Transfer Protein Detection on the Membrane Isoelectric Focusing Theoretical Background Preparation of IEF Gels Isoelectric Focusing in Proteomics Two-dimensional Electrophoresis Sample Preparation Pre-labeling of Proteins for Difference Gel Electrophoresis First Dimension: Isoelectric Focusing in IPG Strips Second Dimension: SDS Electrophoresis Detection of Protein Spots Image Analysis Image Acquisition Image Analysis and Evaluation Use of 2-D Electrophoresis Data Spot Handling Spot Picking Protein Cleavage