2002•Unpublished venueRequires access
Expression Proteomics
Reiner Westermeier, Tom Naven
Open publisher page 7 citations
Abstract
Two-dimensional Electrophoresis Evolution of 2-D methodology Sample preparation First dimension: Isoelectric focusing Second dimension: SDS-PAGE Detection of protein spots Image analysis Spot handling Spot cutting Protein cleavage Mass spectrometry Ionisation Ion separation Tandem mass spectrometry (MS/MS) Protein identification by database searching Protein mass fingerprint Peptide mass fingerprinting combined with composition information Peptide mass fingerprint combined with partial sequence information Product ion MS/MS sequence data De novo sequencing Methods of proteome analysis 2D-MS LC-MS/MS Quantitative proteomics