Inhibitory Activity of Soy Cell Culture Extract on Tyrosinase Activity and Melanin Formation in ⍺ -MSH Induced B16-F10 Melanoma Cells

Abstract Background Hyperpigmentation particularly in the cosmetic industry is one of the most important issue that causes excess production and accumulation of melanin. Nowadays, some materials such as kojic acid and its derivatives and arbutin as a skin whitening are used to overcome hyperpigmentation. Since they have side effects such as skin irritation and contact dermatitis, the new surveys focus on agents which are natural and have no side effect.Methods and Results In the present study, we investigated that effect of soy cell culture extract on α-MSH induced melanogenesis in B16-F10 melanoma cells. We found that the soy cell culture extract supressed melanin sythesis and showed anti tyrosinase activity on B16-F10 melanoma cells in a dose dependent manner without cytotoxicity. In addition, we determined that soy cell culture extract suppressed the expression of TYR, TRP1, TRP2 and MITF at both the gene expression and protein level. Conclusions In conclusion, according to the current results, soy cell culture extract has high potential for using as an ingredient in skin whitening products.