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2010•Proteómica: revista de la Sociedad Española de ProteómicaRequires access

Use of ProteoMiner in Vetenery Research

Anna Marco Ramell, Gemma Rovira, Anna Maria Bassols Teixidó

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Abstract

sis of interacting partners of different proteins that are presented in TERMs [2]. This was accomplished by “pull-down” techniques and high-throughput protein trometry. For this end, synthetic biotinylated peptides spanning the C-terminal cytoplasmic end of these proteins were incubated with extracts from lymphoblast cell models, and then captured using Streptavidin-sepharose microbeads. Proteins interacting with the peptide baits were subjected to digestion and the resulting peptides systematically analyzed by HPLC-linear ion trap MS/MS mass spectrometry. Proteins from more

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sis of interacting partners of different proteins that are presented in TERMs [2]. This was accomplished by “pull-down” techniques and high-throughput protein trometry. For this end, synthetic biotinylated peptides spanning the C-terminal cytoplasmic end of these proteins were incubated with extracts from lymphoblast cell models, and then captured using Streptavidin-sepharose microbeads. Proteins interacting with the peptide baits were subjected to digestion and the resulting peptides systematically analyzed by HPLC-linear ion trap MS/MS mass spectrometry. Proteins from more

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Available abstract

sis of interacting partners of different proteins that are presented in TERMs [2]. This was accomplished by “pull-down” techniques and high-throughput protein trometry. For this end, synthetic biotinylated peptides spanning the C-terminal cytoplasmic end of these proteins were incubated with extracts from lymphoblast cell models, and then captured using Streptavidin-sepharose microbeads. Proteins interacting with the peptide baits were subjected to digestion and the resulting peptides systematically analyzed by HPLC-linear ion trap MS/MS mass spectrometry. Proteins from more

Key concepts: Biotinylation, Streptavidin, Chemistry, Mass spectrometry, Peptide, Sepharose, Chromatography, Cytoplasm

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