The diagnosis and analysis of Cryptosporidium infection in children of Zhejiang province.

Aim To etiological diagnose and analyze of Cryptosporidium infection of diarrhea children in zhejiang province Method 548 fecal specimens were collected from diarrhea children in affiliated children hospital of Zhejiang College Each fecal specimen was smeared and screened by modified acid-fast staining method for oocyst;the positive and suspected specimens were identified by immunofluorescent staining method Result As a result,57 cases of infection were found by modified acid-fast staining and immunofluorescent staining method with a positive rate of 10 4%(57/548) Among the positive specimens 19 cases were under the age of l year,the positive rate was 5 7%(19/332),and 38 cases were between the age 2 and 10 with a positive rate of 17 5%(38/216) Conclusion The best result could be obtained when modified acid-fast staining method was first used and followed by immunofluorescent staining method The study showed that there were high Cryptosporidium infective rate in diarrhea children of Zhejiang province,but the positive rate under the age of 1 year was much lower than older children,this may be due to breast feeding