2005•Unpublished venueRequires access
Role of BRCA2/FANCD1 in Homologous Recombination
Julia Etchin, San Joe Filippo, Patrick Sung, Lumír Krejčí
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Abstract
The molecular function of BRCA2 in homologous recombination has
not been elucidated. We are using biochemical approaches as
well as electron microscopy to validate the hypothesis that
BRCA2 influences Rad51 recombinase activity by nucleating Rad51
onto ssDNA to promote the assembly of the Rad51 presynaptic
filament, a pivotal intermediate in HR reactions. In addition,
we have characterizaed the DNA binding properties of BRCA2-DBD
fragment and examined variants of BRCA2 containing combinations
of the Rad51-interaction and DNA binding motifs for the ability
to target Rad51 to recombination substrates. By deciphering the
functional significance of these interactions, we will be able
to rationalize how mutations in BRCA2 lead to genome
instability and breast cancer.