2009•Chaye kexueRequires access
Effect of theaflavin on the activity of p38 mitogen-activated protein kinase (p38MAPK) and extracellar matrix production in rat mesangial cells.
Cairong Li, Fei Cai, Xinyuan Zhao, Yinqiao Yang
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Abstract
To study the effect of theaflavin on rat mesangial cells(GMCs) were separately incubated with normal glucose,high glucose,advanced glycosylation end products(AGE) and H2O2.The protein expression of p-p38MAPK and TGF-β was examined by Western blot,and the levels of fibronectin(FN),collagen Ⅳ(ColⅣ),laminine(LN) in the supernatant of cultured GMCs were detected by ELISA.Compared to the control group,high glucose,AGE and H2O2 significantly activated p-p38MAPK and increased the protein expression of TGF-β and extracellar matrix production.The protein expression of p-p38MAPK and TGF-β was inhibited by theaflavin.Compared to GMCs incubated with corresponding stimulators,FN,ColⅣ and LN were expressed at a lower level after pre-treatment of theaflavin.It can be concluded that theaflavin may effectively attenuate diabetic nephropathy progression through down-regulating the expression of TGF-β and extracellar matrix production via p38MAPK.