Normal hematological and clinical chemistry blood values in normal long-tailed macaque lived in Mahidol University, Sai-Yok campus, Kanchanaburi.
Arunee Jangsangthong, Shutipen Buranasinsup
Abstract
Arunee Jangsangthong, Shutipen Buranasinsup
Abstract
Investigation of hematology together with blood clinical chemistry is useful for evaluating the health of animals then standard reference range is very essential and should be established. This study investigated the hematology and blood clinical chemistry in 28 normal long-tailed macaques (17 male and 11 female) lived in wild at Mahidol University, Sai-Yok campus, Kanchanaburi. Blood samples were collected and divided into two parts. The EDTA blood was used for hematological analysis whereas the serum from clot blood was used for blood clinical chemistry analysis. Hematology determination was analyzed by using ABX Micros ABC Vet automated hematology analyzer whereas blood clinical chemistry was analyzed by using semi-automated, StarDust MC 15. Sera were used for biochemical enzyme tests including total protein, blood urea nitrogen (BUN), creatinine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). We found that hematology and blood clinical chemistry has normal distribution, no statistically significant difference between males and females, except hemoglobin. In addition, we also performed blood smear and stained with modified Wright's Geimsa for determining red blood cells morphology and white blood cells differential counts.
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Investigation of hematology together with blood clinical chemistry is useful for evaluating the health of animals then standard reference range is very essential and should be established. This study investigated the hematology and blood clinical chemistry in 28 normal long-tailed macaques (17 male and 11 female) lived in wild at Mahidol University, Sai-Yok campus, Kanchanaburi. Blood samples were collected and divided into two parts. The EDTA blood was used for hematological analysis whereas the serum from clot blood was used for blood clinical chemistry analysis. Hematology determination was analyzed by using ABX Micros ABC Vet automated hematology analyzer whereas blood clinical chemistry was analyzed by using semi-automated, StarDust MC 15. Sera were used for biochemical enzyme tests including total protein, blood urea nitrogen (BUN), creatinine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). We found that hematology and blood clinical chemistry has normal distribution, no statistically significant difference between males and females, except hemoglobin. In addition, we also performed blood smear and stained with modified Wright's Geimsa for determining red blood cells morphology and white blood cells differential counts.
Key concepts: Hematology, Creatinine, Internal medicine, Clinical pathology, Blood urea nitrogen, Hematology analyzer, Blood chemistry, Complete blood count