[Research of extracting and purifying taxol from the branches of Taxus media].

OBJECTIVE: The study was to explore extracting and purifying technology of taxol from the branches of Taxus media. METHOD: In extracting phase, the solvent extraction, ultrasonic extraction and the supercritical CO2 fluid extraction were studied respectively; In purifying phase, the extracts were disposed by silica gel column chromatography and preparation lamella chromatography, then crystaled by N-Hexane. The content of taxol was detected by HPLC. RESULT: The results showed that the recovery of extracting toxal with the method of ultrasonic was the highest and the selectivity of supercritical CO2 fluid extraction was the best; The sample after being extracted should be purified two stages by silica gel column chromatography with dichloromethane-chloroform-methane (53:44:3) as elution and the lamella chromatography was chloroform-ethyl acetate-methane (88:7:5) as elution, finally we reached the fawn crystal. CONCLUSION: In that production, the content of taxol reached 87. 3% and the recovery 89.7%, which indicat that the taxol is well enrichment.