Proliferation in vitro of bone marrow-derived mesenchymal stem cells in different culture meeia in rabbits.

Objective To investigate the effects of different culture media on the proliferation in vitro of bone marrow-derived mesenchymal stem cells.Methods Bone marrow was Abstracted from the intertrochanter of the femur of 2-month New Zealand rabbits and purification of mesenchymal stem cells was determined by a density gradient centrifugation method.The cells were cultured in 4 kinds of culture media:DMEM-high glucose(10%FBS),DMEM-low glucose(10%FBS),DMEM-low glucose(15%FBS)and DMEM-low glucose(20%FBS).Cells of passage one were detected the expansion multiple and cells of passage two measured the growth and mineralization of cells.Results The expansion multiple was 16.20±1.60 and the colony-forming number(CFN)was 6.11±1.17 in the DMEM-low glucose(15%FBS)group,which were significantly higher than those in the other three groups.Conclusion DMEM-low glucose(15%FBS)is more suitable for cultivation of MSCs than the other three media.