Cloning and sequence analysis of ras promoter from Auricuralia auricular strain YBS-3.

[Objective] The ras promoters were cloned from genomic DNA of Auricuralia auricular so as to provide promoters for breeding better species by genetic technology.[Method] PCR was used to clone promoters with the genomic DNA of A.auricular strain YBS-3 as template,and then the sequences of four ras promoters were obtained.The analysis of promoter sequences was made by three promoter analysis software:Promoter prediction,Place and TFSEARCH ver.1.3.[Result] Four fragments contained core elements of promoter including TATA-box and CAAT-box,and many other important cis-elements such as GATA BOX,GCGC BOX,CCAAT BOX1,etc.At the same time,there was at least one transcriptional start site in these sequences.And several transcription factor binding sites were detected in these sequences.[Conclusion] Four promoter fragments were all having promoter function theoretically.