Establishment of the AFLP fingerprinting for some tomato cultivars.

The genetic relationship and diversity of 8 tomato germplasm resources which were resistant or suscepti-ble to tomato yellow leaf curl disease(TYLCD) were analyzed by the optimized AFLP(amplified fragments length polymorphism).Five primer combinations selected from 64 primer pairs revealed a total number of 149 unambigu-ous bands,62 of which were polymorphic with a polymorphism frequency of 41.6%.This result showed molecular variations of some enzyme digestion sites among the used tomato germplasm resources.The genetic similarity coeffi-cient of 8 tomato resources ranged from 0.83 to 0.95.Based on the coefficient value of 0.89,these tomato germplasm resources were clustered into 1 multiple-member group and 3 one-member groups by UPGMA(un-weighted pair group method with arithmetic mean) analysis,which provided molecular reference for parent selection in tomato breeding for resistance to TYLCD.