2009•Indian Journal of BiotechnologyRequires access

Micropropagation of Wrightia tomentosa : Effect of gelling agents, carbon source and vessel type

Preeti Joshi, Rohini Trivedi, S. D. Purohit

Abstract

The role of gelling agents, carbon source, type of culture vessel and liquid culture system during rooting phase in Wrightia tomentosa was investigated in an effort to reduce the cost of micropropagation. Studies have revealed significant improvement in shoot multiplication on a medium containing crude agar and sugarcubes. Promotory role of vented vessel in culture growth and liquid culture system during rooting phase was established. The results showed strong potential for reduction in cost of plantlets in vitro.

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Key concepts: Micropropagation, Liquid culture, Tissue culture, Agar, Shoot, Carbon source, Botany, Liquid medium