ScholarLens Back to search
2020•Assiut Veterinary Medical Journal/Maǧallaẗ Asyūṭ al-ṭibiyyaẗ al-baytariyyaẗOpen access

MICROSCOPIC AND MOLECULAR STUDY OF BABESIA SPP. BABESIA BOVIS AND BABESIA BIGEMINA IN MOSUL CITY-IRAQ

Eman Gh. Suleiman, A. F. Al-Taee

Open full text 0 citations

Abstract

The current study included examination of fifty blood samples which collected from both sexes and different ages of cattle in different areas of Mosul city, during the period from October 2017 to the end of November 2017 by using microscopical and polymerase chain reaction (PCR) techniques. The percentage of diagnosis of Babesia spp < /em>. Babesia bovis and Babesia bigemina by (PCR) 60% , 44%, 38% respectively , these percentage were higher than which recorded by microscopic examination 44%, 32%, 32% respectively. The results showed product of amplification on agarose gel 2%, 644, 350, 278 bp respectively, also this study showed that the alternative methods (not commercial) which used for extraction of DNA form the blood of cattle were evaluated as most efficient and obtained good concentration and purity of DNA which used in polymerase chain reaction and these methods characterized as most rapid, simple and dependable methods.

Open-access reader

About this research paper

What this paper is about

The current study included examination of fifty blood samples which collected from both sexes and different ages of cattle in different areas of Mosul city, during the period from October 2017 to the end of November 2017 by using microscopical and polymerase chain reaction (PCR) techniques. The percentage of diagnosis of Babesia spp < /em>. Babesia bovis and Babesia bigemina by (PCR) 60% , 44%, 38% respectively , these percentage were higher than which recorded by microscopic examination 44%, 32%, 32% respectively. The results showed product of amplification on agarose gel 2%, 644, 350, 278 bp respectively, also this study showed that the alternative methods (not commercial) which used for extraction of DNA form the blood of cattle were evaluated as most efficient and obtained good concentration and purity of DNA which used in polymerase chain reaction and these methods characterized as most rapid, simple and dependable methods.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The current study included examination of fifty blood samples which collected from both sexes and different ages of cattle in different areas of Mosul city, during the period from October 2017 to the end of November 2017 by using microscopical and polymerase chain reaction (PCR) techniques. The percentage of diagnosis of Babesia spp < /em>. Babesia bovis and Babesia bigemina by (PCR) 60% , 44%, 38% respectively , these percentage were higher than which recorded by microscopic examination 44%, 32%, 32% respectively. The results showed product of amplification on agarose gel 2%, 644, 350, 278 bp respectively, also this study showed that the alternative methods (not commercial) which used for extraction of DNA form the blood of cattle were evaluated as most efficient and obtained good concentration and purity of DNA which used in polymerase chain reaction and these methods characterized as most rapid, simple and dependable methods.

Key concepts: Babesia bigemina, Babesia bovis, Babesia, Babesiosis, Biology, Virology, Veterinary medicine, Medicine

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
MICROSCOPIC AND MOLECULAR STUDY OF BABESIA SPP. BABESIA BOVIS AND BABESIA BIGEMINA IN MOSUL CITY-IRAQ — Research Paper | ScholarLens