PHYTOCHEMISTRY AND ANTIFEEDANT ACTIVITY OF ROOT EXTRACTS FROM SOME VINCETOXICUM TAXA AGAINST LEPTINOTARSA DECEMLINEATA AND SPODOPTERA LITTORALIS

ABSTRACT Antifeedant activities of dichloromethane, dichloromethane: methanol (1:1), methanol and total ethanol extracts of five Vincetoxicum N.M. Wolf taxa were investigated using leaf disc no-choice method against third stadium larvae of Spodoptera littoralis Bois. and Leptinotarsa decemlineata Say.. Further, phytochemical constituents were also screened qualitatively. Among the 20 tested extracts, 7 extracts against L. decemlineata larvae and 3 extracts against S. littoralis larvae showed 100 % antifeedant activity after exposure maximal dose. The activity results indicated that roots of studied Vincetoxicum taxa were effective against both tested pests. The dichloromethaneand dichloromethane: methanol (1:1) extracts of V. fuscatum subsp. boissieri (Kusn) Browicz indicated the highest effectiveness against L. decemlineata larvae (16 μg /cm2 ED50 value) and CH2Cl2 extract of V. canescens subsp. canescens (Willd.) Decne. showed the highest effectiveness against S. littoralis larvae (ED50 48 μg /cm2 value). Furthermore the growth inhibition and chronic toxicity on S. littoralis larvae were determined. The CH2Cl2 extract of V. fuscatum subsp. boissieri and MeOH: CH2Cl2(1:1) extract of V. canescens subsp. canescens displayed the highest effectiveness causing growth inhibition with ED50 0.04 mg/g and 0.09 mg/g respectively. Also the same extract of V. fuscatum sub-species boissieri produced chronic toxicity with the highest effectiveness letal dose (LD50 1.11 mg/g value). Phytochemical studies showed the presence of steroidal glycosides, sugars and starch. The root of V. fuscatum subsp. boissieri also contained saponins. MS History: 27.10.2015 (Received)-28.10.2015 (Revised)-31.10.2015 (Accepted)