2013•Digital WPIOpen access

Isolation of Mesenchymal Stem Cells from Murine Bone Marrow

Heather Roark Parker

Abstract

Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the potential to differentiate into various cells lineages, including adipocytes, chondrocytes, osteocytes, and fibroblasts. Due to their ability to regenerate damaged tissues, MSCs have the potential to be the future of new cell-based therapies. However, identification of MSCs has not been standardized. The purpose of this project was to identify parameters for isolating MSCs from murine bone marrow. This was accomplished by combining a staining protocol that utilizes the unique surface characteristics of MSCs with the negative selection for CD45 and Ter-119, and then the negative selection for CD44 in the remaining cell population.

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Key concepts: Mesenchymal stem cell, Bone marrow, Stem cell, Isolation (microbiology), Stem cell transplantation for articular cartilage repair, Biology, Cell biology, Adult stem cell