2017UiTM Institutional Repositories (Universiti Teknologi MARA)Open access

In vitro assessment of antioxidant and phytochemical properties of acetone extract leaves from rhodomyrtus tomentosa / Nor Hamidah Ramli

Nor Hamidah Ramli

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Abstract

Rhodomyrtus tomentosa is an ordinary trees, widespread in regions with accessible ground environments and its provide crucial health advantage due to the medicinal value. In this study, Rhodomyrtus tomentosa was evaluated by using 75% acetone extract leaves to determine the phytochemical properties using an in vitro technique that are total phenolic content (TPC) and total flavonoids content (TFC) of acetone extract from Rhodomyrtus tomentosa leaves. The antioxidant activity by measuring the total anti oxidant capacity (TAC) and 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of acetone extract of Rhodomyrtus tomentosa leaves using spectrophotometer. Total phenolic content analyze by using Folin-Ciocalteu and the result was 6.90 ± 1.02 mg gallic acid equivalent/g. Total flavonoids content (TFC) experimental by using aluminium chloride technique was 0.48 ± 0.04 mg quercetin equivalent/g. Spectrophotometric detection of 2,2- diphenyl-1-picrylhydrazyl (DPPH) scavenging acticity was 28.63 ± 31.56 % and total antioxidant capacity marks 5.4 ± 41.85 mg ascorbic acid. The above results revealed antioxidant capacity of 75% acetone extract from Rhodomyrtus tomentosa leaves.

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Rhodomyrtus tomentosa is an ordinary trees, widespread in regions with accessible ground environments and its provide crucial health advantage due to the medicinal value. In this study, Rhodomyrtus tomentosa was evaluated by using 75% acetone extract leaves to determine the phytochemical properties using an in vitro technique that are total phenolic content (TPC) and total flavonoids content (TFC) of acetone extract from Rhodomyrtus tomentosa leaves. The antioxidant activity by measuring the total anti oxidant capacity (TAC) and 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of acetone extract of Rhodomyrtus tomentosa leaves using spectrophotometer. Total phenolic content analyze by using Folin-Ciocalteu and the result was 6.90 ± 1.02 mg gallic acid equivalent/g. Total flavonoids content (TFC) experimental by using aluminium chloride technique was 0.48 ± 0.04 mg quercetin equivalent/g. Spectrophotometric detection of 2,2- diphenyl-1-picrylhydrazyl (DPPH) scavenging acticity was 28.63 ± 31.56 % and total antioxidant capacity marks 5.4 ± 41.85 mg ascorbic acid. The above results revealed antioxidant capacity of 75% acetone extract from Rhodomyrtus tomentosa leaves.

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Available abstract

Rhodomyrtus tomentosa is an ordinary trees, widespread in regions with accessible ground environments and its provide crucial health advantage due to the medicinal value. In this study, Rhodomyrtus tomentosa was evaluated by using 75% acetone extract leaves to determine the phytochemical properties using an in vitro technique that are total phenolic content (TPC) and total flavonoids content (TFC) of acetone extract from Rhodomyrtus tomentosa leaves. The antioxidant activity by measuring the total anti oxidant capacity (TAC) and 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of acetone extract of Rhodomyrtus tomentosa leaves using spectrophotometer. Total phenolic content analyze by using Folin-Ciocalteu and the result was 6.90 ± 1.02 mg gallic acid equivalent/g. Total flavonoids content (TFC) experimental by using aluminium chloride technique was 0.48 ± 0.04 mg quercetin equivalent/g. Spectrophotometric detection of 2,2- diphenyl-1-picrylhydrazyl (DPPH) scavenging acticity was 28.63 ± 31.56 % and total antioxidant capacity marks 5.4 ± 41.85 mg ascorbic acid. The above results revealed antioxidant capacity of 75% acetone extract from Rhodomyrtus tomentosa leaves.

Key concepts: Gallic acid, DPPH, Phytochemical, Acetone, Chemistry, Tannic acid, Ascorbic acid, Antioxidant

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In vitro assessment of antioxidant and phytochemical properties of acetone extract leaves from rhodomyrtus tomentosa / Nor Hamidah Ramli — Research Paper | ScholarLens