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2021•International Journal of Zoological InvestigationsOpen access

Identification and Confirmation of Chironomus circumdatus Kieffer, 1916 (Diptera: Chironomidae) Using DNA Barcoding

Rawal Deepak, Verma Himani, Prajapat Gyata

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Abstract

Present study was performed to identify and confirm collected larval Chironomid up to species level. To assess molecular taxonomy and phylogeny, DNA barcoding was done using Sanger dideoxy sequencing of mitochondrial cytochrome c oxidase subunit I (COI) gene of larval sample. Bioinformatics analysis was done by using NCBI’s BLAST software. By evidence of DNA barcoding, it was confirmed that present larval chironomid species was Chironomus circumdatus Kieffer, 1916.

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What this paper is about

Present study was performed to identify and confirm collected larval Chironomid up to species level. To assess molecular taxonomy and phylogeny, DNA barcoding was done using Sanger dideoxy sequencing of mitochondrial cytochrome c oxidase subunit I (COI) gene of larval sample. Bioinformatics analysis was done by using NCBI’s BLAST software. By evidence of DNA barcoding, it was confirmed that present larval chironomid species was Chironomus circumdatus Kieffer, 1916.

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Available abstract

Present study was performed to identify and confirm collected larval Chironomid up to species level. To assess molecular taxonomy and phylogeny, DNA barcoding was done using Sanger dideoxy sequencing of mitochondrial cytochrome c oxidase subunit I (COI) gene of larval sample. Bioinformatics analysis was done by using NCBI’s BLAST software. By evidence of DNA barcoding, it was confirmed that present larval chironomid species was Chironomus circumdatus Kieffer, 1916.

Key concepts: DNA barcoding, Chironomidae, Chironomus, Biology, Mitochondrial DNA, Cytochrome c oxidase subunit I, Taxonomy (biology), Molecular taxonomy

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Identification and Confirmation of Chironomus circumdatus Kieffer, 1916 (Diptera: Chironomidae) Using DNA Barcoding — Research Paper | ScholarLens