IMPROVED AND EFFICIENT PROTOCOL FOR DIRECT REGENERATION FROM COTYLEDONARY NODE OF GROUNDNUT (ARACHIS HYPOGAEA L.)

Protocol has been developed for micropropagation of Arachis hypogaea L. under in vitro conditions. Cotyledonary node explants gave rise to multiple shoots when cultured on MS (Murashige and Skoog) medium supplemented with different concentrations of TDZ, BAP and Kin (Kinetin) singly along with auxin. The highest response of shoot multiplication was obtained in MS containing 1.0 mg/l TDZ and 0.5 mg/l NAA. Multiple shoots were transfer to elongation medium augmented with Gibberellic acid (GA3) + Indole butyric acid (IBA). The regenerated shoot lets were rooted on MS basal medium with different concentrations of IBA (Indole butyric acid) and NAA (Naphthalene acetic acid). The maximum frequency of rooting and highest number of roots were produced on medium containing IBA 0.5 mg/l. The plantlets, thus successfully established in soil and later to field.