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HPLC를 이용한 진피(陳皮)의 분석 방법 연구

조성원, 홍선표, 최호영, 최환수, 이제현

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Abstract

Objective : Citrus unshiu Markovich(Rutaceae) has been used in traditional medicine as a digestant, expectorant, and in the treatment of diarrhea, anal prolapes. Hesperidin, naringin contents of citrus fruits have been determined. Methods : The Citrus unshiu was dried in dry oven. The extraction was sonicated in 100% methanol in respectively. A quantitative analysis of hesperidin, naringin using HPLC method. Analysis of naringin, hesperidin, neohesperidin, narigenin and hesperetin was conducted by HPLC with reversed-phase C18 column and a UV detecter at 204 nm. Elution was carried out at 1.0 ml/min with 38% methanol. Result and Conclusion : Sonication in 100% methanol for 1.5hr was the better extracting method that can extracted naringin and hesperidin at the same time. And 38% methanol of mobile phase was good resolution and could analyze into naringin and hesperidin at the same time.

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Objective : Citrus unshiu Markovich(Rutaceae) has been used in traditional medicine as a digestant, expectorant, and in the treatment of diarrhea, anal prolapes. Hesperidin, naringin contents of citrus fruits have been determined. Methods : The Citrus unshiu was dried in dry oven. The extraction was sonicated in 100% methanol in respectively. A quantitative analysis of hesperidin, naringin using HPLC method. Analysis of naringin, hesperidin, neohesperidin, narigenin and hesperetin was conducted by HPLC with reversed-phase C18 column and a UV detecter at 204 nm. Elution was carried out at 1.0 ml/min with 38% methanol. Result and Conclusion : Sonication in 100% methanol for 1.5hr was the better extracting method that can extracted naringin and hesperidin at the same time. And 38% methanol of mobile phase was good resolution and could analyze into naringin and hesperidin at the same time.

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Available abstract

Objective : Citrus unshiu Markovich(Rutaceae) has been used in traditional medicine as a digestant, expectorant, and in the treatment of diarrhea, anal prolapes. Hesperidin, naringin contents of citrus fruits have been determined. Methods : The Citrus unshiu was dried in dry oven. The extraction was sonicated in 100% methanol in respectively. A quantitative analysis of hesperidin, naringin using HPLC method. Analysis of naringin, hesperidin, neohesperidin, narigenin and hesperetin was conducted by HPLC with reversed-phase C18 column and a UV detecter at 204 nm. Elution was carried out at 1.0 ml/min with 38% methanol. Result and Conclusion : Sonication in 100% methanol for 1.5hr was the better extracting method that can extracted naringin and hesperidin at the same time. And 38% methanol of mobile phase was good resolution and could analyze into naringin and hesperidin at the same time.

Key concepts: Naringin, Hesperidin, Citrus unshiu, Chemistry, Chromatography, High-performance liquid chromatography, Methanol, Organic chemistry

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HPLC를 이용한 진피(陳皮)의 분석 방법 연구 — Research Paper | ScholarLens