Molecular Detection of Aggregatibacter actinomycetemcometans and Porphyromonas gingivalis in Children with Periodontal Disease

Objective: The aim of the present study was to evaluate the presence of Aggregatibacter actinomycetemcomitans (A.actinomycetemcomitans) and Porphyromonas gingivalis (P.gingivalis) in healthy and different stage of gingivitis for children aged between 9 and 12 years using Polymerase Chain Reaction (PCR) and to compare their presence in children in their healthy, mild and severe gingivitis.Method: Subgingival plaque samples were collected from 94 children and were grouped as Group A-control (healthy), Group B-mild gingivitis, Group C moderate gingivitis, and Group D sever gingivitis and were subjected to PCR assay.Results: All data were analyzed by using SPSS program (version 21 for windows 7) chi-square test at p≤0.05. A. actinomycetemcomitans was detected in 0 (0%), 4 (13.4%),37 (92.5%) and 12 (100%) whereas P. gingivalis was detected in 0 (0%), 0 (0%), 6 (15%) and 0 (0%) samples in group A, B, C and D respectively Conclusion: There is nor A. actinomycetemcomitans or Porphyromonas gingivalis found in group A (healthy moth children).Both the microorganisms were present in group C. In group B and D only A. actinomycetemcomitans was present.