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2017•Assiut Veterinary Medical Journal/Maǧallaẗ Asyūṭ al-ṭibiyyaẗ al-baytariyyaẗOpen access

OCCURRENCE OF CAMPYLOBACTER SPECIES IN CHICKENS BY MULTIPLEX POLYMERASE CHAIN REACTION

Asmaa Gahlan Youseef, I. A. Ibrahim, Amal S. M. Sayed, Mona Sobhy

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Abstract

The genus Campylobacter is one of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75) chickens including intestinal content (75), liver (75) and skin (75). The overall occurrence of Campylobacter jejuni and Campylobacter coli in chicken by PCR were (5.3% & 17.8%). Multiplex PCR targeting 23S rRNA specific for genus Campylobacter, hip O gene specific for C. jejuni and glyA gene specific for C. coli was used for the confirmation of phenotypically identified C. jejuni and C. coli isolates. It is concluded that PCR was determined to be more specific and rapid than biochemical tests.

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The genus Campylobacter is one of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75) chickens including intestinal content (75), liver (75) and skin (75). The overall occurrence of Campylobacter jejuni and Campylobacter coli in chicken by PCR were (5.3% & 17.8%). Multiplex PCR targeting 23S rRNA specific for genus Campylobacter, hip O gene specific for C. jejuni and glyA gene specific for C. coli was used for the confirmation of phenotypically identified C. jejuni and C. coli isolates. It is concluded that PCR was determined to be more specific and rapid than biochemical tests.

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Available abstract

The genus Campylobacter is one of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75) chickens including intestinal content (75), liver (75) and skin (75). The overall occurrence of Campylobacter jejuni and Campylobacter coli in chicken by PCR were (5.3% & 17.8%). Multiplex PCR targeting 23S rRNA specific for genus Campylobacter, hip O gene specific for C. jejuni and glyA gene specific for C. coli was used for the confirmation of phenotypically identified C. jejuni and C. coli isolates. It is concluded that PCR was determined to be more specific and rapid than biochemical tests.

Key concepts: Campylobacter, Polymerase chain reaction, Multiplex polymerase chain reaction, Biology, Multiplex, Microbiology, Genetics, Bacteria

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OCCURRENCE OF CAMPYLOBACTER SPECIES IN CHICKENS BY MULTIPLEX POLYMERASE CHAIN REACTION — Research Paper | ScholarLens