Establishment of Optimal Storage Temperature after Frozen-thawed of Black Goat Epididymal Spermatozoa.

The purpose of this study was to observe the changes in motility after frozen-thawing of black goat epididymal sperm. The black goat testis was transported to the laboratory within 30 minutes from the slaughterhouse and the temperature was set at 36.5℃. Sperm were collected by pressing the sliced epididymis and used the Semen Washing Media. The egg yolk-triladyl frozen solution was used for the freezing of black goat sperm and the freezing concentration was set to 1×108 sperm/mL. The frozen sperm were thawed in water bath at 37.5℃ for 45 seconds and motility was measured after preservation for 0, 30, 60, 90 and 120 min at 4℃, 17℃ and 37℃, respectively. In results, sperm motility at 30 min after thawing was significantly higher in 17℃ than 37℃ (50.44% vs. 26.60%; p<0.05). Likewise, sperm motility at storage time 60, 90, and 120 min after thawing was significantly lower in frozen-thawed sperm stored at 37℃ groups than frozen-thawed sperm stored at 4℃ and 17℃ (p<0.05). Although there was no significant difference between frozen-thawed sperm stored at 4℃ and 17℃, motility in the frozen-thawed sperm stored at 17℃ was slightly higher than stored at 4℃. In conclusion, this results suggested that 4℃ and 17℃ temperature are more efficient for storage of frozen-thawed spermatozoa in black goat than 37℃. However, more researches are needed to establish the optimal conditions or proper supplementation for sperm preservation.