Detection of Vector Mosquito of Filariasis in the Endemic Areas of Bangladesh

Background: Lymphatic Filariasis (LF), a mosquito born tropical parasitic disease, caused by Wuchereria bancrofti is not only a health but also a socio-economic problem in Bangladesh. Culex quinquefasciatus is the confirmed filarial vector in Bangladesh, so by controlling of this vector population, we can prevent this disease. To control this vector and decrease incidence of filariasis, we need to know studied mosquito’s sample in endemic areas. Aim and objectives: Aim of this study was to see mosquito population in search of vector species in the endemic zones of malaria and filarial. Then we confirmed presence of vector species at molecular level by species specific DNA fingerprinting. Methods: This cross-sectional entomological study was carried out in Hobiganj (Shatchori tea garden) and Moulvibazar district (Patrokhola and Madonmohonpur tea gardens). Mosquito was collected by paper cup with net, aspirator and torch light and vector mosquitoes were identified with stereoscopic microscope. After identification of the mosquitoes, these were stored in laboratory for analysis of the density of the vector mosquitoes in the endemic areas. Then the vector mosquitoes were identified through molecular method named Polymerase Chain Reaction (PCR). Result: 1427 female mosquitoes belonging to 28 species under 5 genera were collected from three tea gardens. Culex quinquefasciatus which is the confirmed filarial vector in Bangladesh was found high in number (20.74%) out of the total collected mosquitoes. Other mosquito’s species were found in various ratios. Then Culex quinquefasciatus vector was identified by PCR. Conclusion: As vector mosquitoes of LF are available in these tea gardens, the study concludes that these areas are highly LF endemic areas of Bangladesh. Through molecular method, the vector of LF can be identified certainly. As we identify the vector mosquito, thus we can prevent this LF disease at very early stage. So, these findings will be very beneficial and cost effective for a developing country like Bangladesh.