2019Problems of Veterinary Sanitation Hygiene and EcologyRequires access

VERIFICATION OF THE METHOD OF ISOLATION OF VIBRIO PARAHAEMOLYTICUS FROM SEA FISH AND FISH PRODUCTS

I.M. Nityaga, Б. В. Уша, O. V. Prostova

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Abstract

Analysis of microbiological safety of fish and fish products involves the assessment of the presence of a number of pathogenic bacteria in these products. One of these important bacteria is Vibrio parahaemolyticus – a gram-negative halophilic bacilus found in warm sea waters. The aim of this work was to verify the method of isolation of Vibrio parahaemolyticus from marine fish and fish products, as well as to study the presence of Vibrio samples of fish products from large retail chains. Verification of the method of detection of parahemolytic Vibrio from fish and fish products was carried out in accordance with GOST ISO/TS 21872-2-2013 "Microbiology of food and animal feed. Horizontal method for detection of potentially enteropathogenic Vibrio spp. Part 2. "Detection of bacteria species differs from Vibrio parahaemolyticus and Vibrio cholerae". As a result of the performed studies, the reliability of this method of detection of Vibrio parahaemolyticus on artificially contaminated samples was established. During monitoring fish products, parahemolytic vibrio was not detected.

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What this paper is about

Analysis of microbiological safety of fish and fish products involves the assessment of the presence of a number of pathogenic bacteria in these products. One of these important bacteria is Vibrio parahaemolyticus – a gram-negative halophilic bacilus found in warm sea waters. The aim of this work was to verify the method of isolation of Vibrio parahaemolyticus from marine fish and fish products, as well as to study the presence of Vibrio samples of fish products from large retail chains. Verification of the method of detection of parahemolytic Vibrio from fish and fish products was carried out in accordance with GOST ISO/TS 21872-2-2013 "Microbiology of food and animal feed. Horizontal method for detection of potentially enteropathogenic Vibrio spp. Part 2. "Detection of bacteria species differs from Vibrio parahaemolyticus and Vibrio cholerae". As a result of the performed studies, the reliability of this method of detection of Vibrio parahaemolyticus on artificially contaminated samples was established. During monitoring fish products, parahemolytic vibrio was not detected.

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Available abstract

Analysis of microbiological safety of fish and fish products involves the assessment of the presence of a number of pathogenic bacteria in these products. One of these important bacteria is Vibrio parahaemolyticus – a gram-negative halophilic bacilus found in warm sea waters. The aim of this work was to verify the method of isolation of Vibrio parahaemolyticus from marine fish and fish products, as well as to study the presence of Vibrio samples of fish products from large retail chains. Verification of the method of detection of parahemolytic Vibrio from fish and fish products was carried out in accordance with GOST ISO/TS 21872-2-2013 "Microbiology of food and animal feed. Horizontal method for detection of potentially enteropathogenic Vibrio spp. Part 2. "Detection of bacteria species differs from Vibrio parahaemolyticus and Vibrio cholerae". As a result of the performed studies, the reliability of this method of detection of Vibrio parahaemolyticus on artificially contaminated samples was established. During monitoring fish products, parahemolytic vibrio was not detected.

Key concepts: Vibrio parahaemolyticus, Vibrio, Microbiology, Biology, Fish <Actinopterygii>, Isolation (microbiology), Vibrio cholerae, Vibrio Infections

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